PUBLICATIONS
IBA PUBLICATIONS
Miraldo A, Iwaszkiewicz-Eggebrecht E, Buczek M, Goodsell R, Johansson H, Fisher BL, et al. Data of the Insect Biome Atlas: a metabarcoding survey of the terrestrial arthropods of Sweden and Madagascar. bioRxiv. Published online October 2024. https://doi.org/10.1101/2024.10.24.619818
Sundh J, Granqvist E, Iwaszkiewicz-Eggebrecht E, Manoharan L, van Dik L, Goodsell R, Lukasik P, Miraldo A, Roslin T, et al. HAPP: High-Accuracy Pipeline for Processing of deep metabarcoding data. Published online 2024. bioRxiv. https://doi.org/10.1101/2024.12.20.629441
Iwaszkiewicz-Eggebrecht E, Goodsell RM, Bengsson BÅ, Mutanen M, Klinth M, van Dijk L, Lukasik P, Miraldo A, et al. High-throughput biodiversity surveying sheds new light on the brightest of insect taxa. bioRxiv. Published online October 2024. https://doi.org/10.1101/2024.10.25.620209
van Dijk LJA, Miraldo, A, Raharinjanahary D, Rajoelison ET, Fisher BL, Goodsell RM, Iwaszkiewicz-Eggebrecht E, Åhlén D, Högvall J, Lundberg E, Rova E, Łukasik P, Ronquist F, Roslin T, Tack AJM. 2024. Biotic and abiotic drivers of ecosystem functioning differ between a temperate and a tropical region. bioRxiv. https://doi.org/10.1101/2024.02.28.582312
Goodsell R, Tack A, Ronquist F, van Dijk LVA, Iwaszkiewicz-Eggebrecht E, Miraldo A, Roslin T, Vanhatalo J. 2024. Moving towards better risk assessment for invertebrate conservation. EcoEvoRxiv. https://doi.org/10.32942/X23G71
van Dijk LA, Fisher B, Miraldo A, Goodsell R, Iwaszkiewicz-Eggebrecht E, Raharinjanahary D, Rajoelison ET, Łukasik P, Andersson A, Ronquist F, Roslin T, Tack A. 2024. Temperature and water availability drive insect seasonality across temperate and tropical climates. Proceedings of the Royal Society B. 291: 20240090. https://doi.org/10.1098/rspb.2024.0090
Iwaszkiewicz-Eggebrecht E, Zizka V, Lynggaard C, 2024. Three steps towards comparability and standardization among molecular methods for characterizing insect communities. Philos. Trans. R. Soc. B Biol. Sci. 379, 20230118. https://doi.org/10.1098/rstb.2023.0118
Iwaszkiewicz-Eggebrecht E, Granqvist E, Buczek M, Prus M, Kudlicka J, Roslin T, Tack AJM, Andersson AF, Miraldo A, Ronquist F, Łukasik P, 2023. Optimizing insect metabarcoding using replicated mock communities. Methods Ecol. Evol. 14, 1130–1146. https://doi.org/10.1111/2041-210X.14073
Iwaszkiewicz-Eggebrecht E, Łukasik P, Buczek M, Deng J, Hartop EA, Havnås H, Prus-Frankowska M, Ugarph CR, Viteri P, Andersson AF, Roslin T, Tack AJM, Ronquist F, Miraldo A. 2023. FAVIS: Fast and versatile protocol for non-destructive metabarcoding of bulk insect samples. PLOS ONE 18, e0286272. https://doi.org/10.1371/journal.pone.0286272
van Klink R, August T, Bas Y, Bodesheim P, Bonn A, Fossøy F, Høye TT, Jongejans E, Menz MHM, Miraldo A, Roslin T, Roy HE, Ruczyński I, Schigel D, Schäffler L, Sheard JK, Svenningsen C, Tschan GF, Wäldchen J, Zizka VMA, Åström J, Bowler DE. 2022. Emerging technologies revolutionise insect ecology and monitoring. Trends Ecol. Evol. 37, 872–885. https://doi.org/10.1016/j.tree.2022.06.001
Marquina D, Buczek M, Ronquist F, Łukasik P. 2021.The effect of ethanol concentration on the morphological and molecular preservation of insects for biodiversity studies. PeerJ. https://doi.org/10.7717/peerj.10799
Arribas P, Andújar C, Bidartondo MI, Bohmann K, Coissac É. et al. 2021. Connecting high‐throughput biodiversity inventories: Opportunities for a site‐based genomic framework for global integration and synthesis. Molecular Ecology, https://doi.org/10.1111/mec.15797
PROTOCOLS DEVELOPED BY IBA
IBA has developed several lab protocols for processing bulk insect samples to use in metabarcoding. We make all our protocols publicly available through the links below.
FAVIS - https://www.protocols.io/view/favis-fast-and-versatile-protocol-for-metabarcodin-ce24tggw.html
Homogenization - https://www.protocols.io/view/sample-homogenization-and-dna-extraction-for-bulk-yxmvm31bbl3p/v1
Spike-ins - https://www.protocols.io/view/synthetic-coi-spike-ins-for-use-in-metabarcoding-b-14egn33ryl5d/v2
OTHER RELEVANT PUBLICATIONS
van Klink, R., Sheard, J.K., Høye, T.T., Roslin, T., Do Nascimento, L.A., Bauer, S., 2024. Towards a toolkit for global insect biodiversity monitoring. Philos. Trans. R. Soc. B Biol. Sci. 379, 20230101. https://doi.org/10.1098/rstb.2023.0101
Karlsson D., Forshage M., Holston K. & Ronquist F. 2020. The data of the Swedish Malaise Trap Project, a countrywide inventory of Sweden's insect fauna. Biodiversity Data Journal, https://doi.org/10.3897/BDJ.8.e56286
Karlsson D., Hartop E., Forshage M., Jaschhof M. & Ronquist F. 2020. The Swedish Malaise trap project: a 15 year retrospective on a countrywide insect inventory. Biodiversity Data Journal, https://doi.org/10.3897/BDJ.8.e47255
Ronquist F., Forshage M., Häggqvist S., Karlsson D., Hovmöller R., et al. 2020. Completing Linnaeus’s inventory of the Swedish insect fauna: Only 5,000 species left?. PLOS ONE, https://doi.org/10.1371/journal.pone.0228561
Hugerth L. W. & Andersson A. F. 2017. Analysing Microbial Community Composition through Amplicon Sequencing: From Sampling to Hypothesis Testing. Frontiers in microbiology, https://doi.org/10.3389/fmicb.2017.01561
Abdelfattah A., Wisniewski M., Schena L. & Tack A. J. M. 2021. Experimental evidence of microbial inheritance in plants and transmission routes from seed to phyllosphere and root. Environmental Microbiology, https://doi.org/10.1111/1462-2920.15392
Blanchet F. G., Roslin T., Kimura M. T., Kaartinen R., Gripenberg S. & Tack A. J. M. 2018. Related herbivore species show similar temporal dynamics. Journal of Animal Ecology, https://doi.org/10.1111/1365-2656.12807
Ji Y., Huotari T., Roslin T., Schmidt N. M., Wang J. et al. 2020. SPIKEPIPE: A metagenomic pipeline for the accurate quantification of eukaryotic species occurrences and intraspecific abundance change using DNA barcodes or mitogenomes. Molecular Ecology Resources, https://doi.org/10.1111/1755-0998.13057
Marquina D., Anderssson A. & Ronquist F. 2019. New mitochondrial primers for metabarcoding of insects, designed and evaluated using in silico methods. Molecular Ecology Resources, https://doi.org/10.1111/1755-0998.12942
Roslin T. V., Traugott M., Jonsson M., Creer S. & Symondson, W. O. C. 2019. Special issue on species interactions, ecological networks and community dynamics: untangling the entangled bank using molecular techniques. Molecular Ecology, https://doi.org/10.1111/mec.14974